Fusarium oxysporum CRISPR gene editing as novel materials for plant resilience

Project Overview

This three-year research project develops an innovative CRISPR gene editing platform to transform pathogenic Fusarium oxysporum strains into beneficial biological control agents. By systematically removing key virulence genes, we aim to create non-pathogenic fungal strains capable of providing cross-protection against soil-borne plant diseases. The project employs advanced ribonucleoprotein delivery systems and optimised transformation protocols to achieve precise genetic modifications whilst maintaining the fungus’s competitive colonisation abilities. This biotechnology approach offers sustainable alternatives to chemical pesticides, addressing critical agricultural challenges through environmentally responsible crop protection strategies. The research establishes foundational technologies for next-generation biological disease management systems with broad agricultural applications.

Project Aims

• Establish an optimised CRISPR gene editing platform for Fusarium oxysporum
• Create non-pathogenic Fusarium oxysporum strains through targeted gene knockout
• Validate cross-protection efficacy and elucidate protective mechanisms